Objective:

To enhance mass spectrometry imaging by developing a nanostructured surface that improves adaptability and performance across various sample types.

Key Findings:

• The new platform allows for cleaner low-mass spectra and stronger ion signals.
• It is compatible with diverse sample preparation workflows.
• The method successfully mapped neonicotinoid pollutants and metabolites in cowpea seedlings.
• Achieved 10-micrometer-resolution lipid maps from stamped fingerprints.
• Resolved lipid patterns and metabolic changes in cryo-sectioned mouse and rat brains linked to focal cerebral ischemia.

Interpretation:

The substrate's effectiveness is attributed to the synergistic effects of plasmonic enhancement from gold nanoparticles, charge-transfer from the COF, and the structural support from the nanotube scaffold.

Limitations:

Conclusion:

This advancement in mass spectrometry imaging brings it closer to being a universal tool for analyzing complex biological samples.