5 Key Takeaways

• 1

  Low-dose exposure to PFAS alters cellular metabolism despite minimal intracellular accumulation.

• 2

  The study utilized a long-term cell culture model to assess the effects of PFOA and PFOS on human epithelial cells.

• 3

  PFOS levels increased rapidly within 24 hours but stabilized, while PFOA remained below detection limits throughout.

• 4

  Transcriptomic and lipidomic analyses revealed changes in oxidative stress pathways and lipid metabolism due to PFAS exposure.

• 5

  PFAS toxicity may arise from interactions with cellular membranes rather than sustained intracellular accumulation.