Objective:

To evaluate the effectiveness of LEGO blocks as standard samples for fluorescence avoidance in Raman spectroscopy and explore the potential of SPAD technology in addressing fluorescence interference.

Key Findings:

• LEGO blocks are effective standard samples due to their reproducibility, stability, and range of colors, addressing a critical gap in fluorescence mitigation.
• Longer wavelength excitation generally yields better spectral quality in handheld Raman instruments, highlighting the need for optimized excitation strategies.
• SPAD technology shows promise for fluorescence discrimination but did not outperform 1064 nm excitation in current tests, indicating areas for future improvement.

Interpretation:

The findings suggest that LEGO blocks can significantly enhance the evaluation of fluorescence mitigation strategies in Raman spectroscopy, while SPAD technology may offer future advancements despite current limitations, warranting further exploration.

Limitations:

• Current SPAD arrays have limitations in performance compared to established methods, which may hinder their immediate application.
• The study's findings may not generalize to all types of samples or instruments, necessitating caution in broader applications.

Conclusion:

LEGO blocks provide a novel and effective approach to standardization in Raman spectroscopy, and SPAD technology holds potential for future applications despite current challenges, paving the way for advancements in the field.