5 Key Takeaways

• 1

  Interest in supercritical fluid chromatography (SFC) has increased due to new generation systems that enhance sensitivity and robustness.

• 2

  Ultra-high performance SFC (UHPSFC) systems show improved reliability and performance, although extra-column band broadening remains a concern.

• 3

  The study by De Pauw et al. quantified contributions to extra-column band broadening, emphasizing the importance of UV cell volume.

• 4

  Minimizing tubing ID in SFC did not significantly improve plate count, contrasting with traditional liquid chromatography practices.

• 5

  Future UHPSFC systems should optimize compatibility with narrow bore columns and develop UV cells with less than 1 µL volume.