Peptide Mapping uses UHPLC to check the constituent amino acids by comparing the pattern of the peptide fragment chromatogram after the enzyme digestion of protein. Recently, this method has also been applied to quality control testing of bio-pharmaceutical therapies. As a result, it is important that the chromatography is of high resolution and high speed with very low variability. Here, the high resolution, high speed measurement result using the ChromasterUltraRs is introduced.BSA digest was used as model sample, and a comparison was made between conventional and high resolution columns. The peak capacity (4σ) for each column was then compared.
Such a Peptide Mapping method is specified as a test method following the harmonization of three pharmacopoeias, namely JP, EP and USP.
It was calculated from the results that the new high resolution, high speed analysis with the LaChromUltra II column and ChromasterUltraRs UHPLC system gives 1.4 times better peak capacity and half the analysis time compared to the chromatogram using a column with 5 μm particle size.
The ChromasterUltraRs is an excellent tool for peptide mapping applications owing to its highly stable pump technology, very low carry over autosampler and ultra-sensitive detector flow cell. Drawing on 30 years of engineering highly robust and durable HPLC systems, the ChromasterUltraRs is based on the renowned high quality engineering of Hitachi with many new innovations that improve reliability of results, laboratory work flow and method transfer.
To book a demonstration of this outstanding new system, please email us at [email protected] or contact your local VWR representative. We look forward to hearing from you.
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