LC-MS/MS Method for the Determination of Diclofenac in Human Plasma

SOLA products revolutionize solid phase extraction (SPE). This first-in-class SPE product range introduces next generation, innovative technological advancements that give unparalleled performance compared to conventional SPE, phospholipid and protein precipitation products. This includes: 

• Higher levels of reproducibility 
• Higher levels of extract cleanliness 
• Lower elution volumes
• Increased sensitivity 

SOLA products have significant advantages for the analyst when processing compounds in complex matrices, particularly in high-throughput bioanalytical laboratories where reduced failure rates, higher analysis speed and lower solvent requirements are critical. The increased performance gives higher confidence in analytical results and lowers cost without compromising ease of use or requiring complex method development.  Accucore HPLC columns use Core Enhanced Technology™ to facilitate fast and highly efficient separations.  The 2.6 μm diameter particles are not totally porous but have a solid core and a porous outer layer. The optimized phase bonding creates a series of high-coverage, robust phases. Accucore RP-MS uses an optimized alkyl chain length for more effective coverage of the silica surface. This coverage results in a significant reduction in secondary interactions and highly efficient peaks with very low tailing. The tightly controlled  2.6 μm diameter of the Accucore particles results in much lower backpressures than typically seen with sub-2 μm materials.

Extracted diclofenac standards from human plasma gave a linear calibration curve over the dynamic range of 1 to 1000 ng/mL with an r2 coefficient of 0.999 (Figure 1). Six replicates of a 15ng/mL Quality Control (QC) sample were analysed. The precision of the QC was less than 4.4% CV (Table 1). Overspikes were run in triplicate at a concentration of 15 ng/mL and used to calculate the 85.8% percentage recovery level for diclofenac.

Figure 1: Diclofenac linearity over the dynamic range 1 to 1000 ng/mL

SOLA SPE in combination with Accucore RP-MS columns allow for a simple extraction and quantification of diclofenac from human plasma reaching the desired lower limit of quantitation of 1 ng/mL. Extraction recovery was suitably high and the method showed excellent precision with a percentage coefficient of variation of less than five percent (n=6). 

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